Service Details
DNA/RNA extraction
We perform extractions of DNA and RNA (total RNA) from blood, plasma, serum, tissue, cells, etc. If necessary, concentration and quality of extracted/purified DNA/RNA is evaluated. After confirming the validity of the DNA/RNA, the next step of determination is conducted.
We can provide any method of extraction/purification and determination of concentration/quality, from general methods using commercial kits and client specified methods.
Gene expression analysis
We will propose the best method among various analytical platforms according to the type and number of Gene of Interest (GOI) to analyze.
Gene expression profiling by microarray
For comprehensive analysis of gene expression, we provide contract services utilizing GeneChip from Agilent and Affymetrix (currently: Thermo Fisher Scientific). A wide variety of high-quality microarrays are available to meet our clients’ needs, including microarrays for analyzing miRNA.
Mediford Corporation is certified as a contract service provider for Affymetrix.
Gene expression analysis by RT-qPCR
We provide analysis services by Reverse transcription-quantitative PCR (RT-qPCR) to determine expression levels of specific genes. It is effective for analysis of specific gene sequences, specific biological pathways, biological processes, and gene groups related to diseases.
In the field of gene/cell therapy in which development is increasing in recent years, real-time PCR can be used for Determination of Drug Concentrations in vivo. We have an increasing track record in in-vivo determination of CAR loading cells such as CAR-T and recombinant viruses.
We can comply with the reliability standard in expression level analysis of specific genes.
Gene expression analysis by digital omics analyzer
We also support direct digital count of nucleic acids without PCR multiplication. nCounter Analysis system from Bruker Spatial Biology is an analysis device which can count nucleic acid level digitally without PCR multiplication by adding a special molecular barcode. It is possible to analyze up to 800 genes simultaneously. Accurate and reproducible results can be obtained from various matrices, from blood to FFPE tissue.
We can support a series of applications such as RNA analysis, DNA analysis, and miRNA analysis. We have catalogue panels for mice and rats in addition to human panels, and custom designed panels are also available. We can conduct analyses complying with reliability standards.
Gene mutation analysis
Gene mutation analysis by genotyping array
Comprehensive SNPs analysis in genome DNA is available using GeneChip Genotyping array from Affymetrix (currently: Thermo Fisher Scientific).
Gene mutation analysis by PCR
PCR based mutation analysis of specific genome region is available. We can conduct special analysis such as comparison between normal tissue and tumor tissue macro-dissected from a histopathological section.
Pharmacogenomics analysis
Analyzing gene mutation coding drug metabolism and drug transporters is expected to improve safety and efficacy of dosing drugs in drug development.
Analytical services with pharmacogenomics solution from Thermo Fisher Scientific is available. For example, Axiom PharmacoScan Array can conduct comprehensive genotyping by analyzing approximately 1,200 genes and 4,627 drug adsorption/distribution/metabolism/excretion (ADME) markers simultaneously.
