Analytical Instruments

Cell Characterization

Flow Cytometer

Manufacturer
Japan BD
Model
BD FACSLyric Flow Cytometer
Instrument
Specifications
  • 3 laser, 12 color measurement (5-4-3)
  • Medical device manufacturing and marketing notification number: 13B1X10407000161
Measurement
Applications

This system is compatible with high-brightness fluorescent dyes developed by BD, making it ideal for detecting antigens with low expression levels. It enables the rapid measurement of large numbers of cells, processing up to 35,000 events per second, and allows for the assessment of rare cell populations. In addition to the Lyric, Mediford Corporation also owns a FACSCanto II (capable of analyzing eight colors).

Cell Sorter

Manufacturer
BD Biosciences
Model
BD FACSAria Fusion Cell Sorter
Measurement
Applications

By irradiating stained cells, chromosomes, microbes, etc. with laser light, the system allows for the rapid and high-purity collection of specific cells within a cell population while keeping them alive.

CyTOF Mass Cytometer

Manufacturer
Standard BioTools K.K.
Model
CyTOF XT system
Instrument
Specifications
  • Channels: 135
  • Mass range: 75-209 amu
  • Dynamic range: 4.5 orders of magnitude
Measurement
Applications

This is a flow cytometer that uses the principles of ICP-TOF-MS. As metal mass instead of fluorescence is measured, there is zero signal overlap. This allows for the simultaneous measurement of markers for more than 40 parameters, enabling detailed analysis of immune cells.

ImmunoSpot Analyzer

Manufacturer
Cellular Technology, LTD
Model
S6 Core
Instrument
Specifications
  • Visible light
  • Analysis parameters: 2
Measurement
Applications
  • ELISpot
  • Tumor immunity and infection immunity analysis

This is the device used for ELISPOT assay. It detects cells that specifically react to antigens such as vaccines. Although IFN-γ is often used as the sole indicator to evaluate cellular immunity, it is also possible to measure other indicators such as IL-4 at the same time. Humoral immunity can also be evaluated by detecting specific antibodies.

High-Content Analysis System

Manufacturer
Thermo Fisher Scientific K.K
Model
CellInsight CX5
Instrument
Specifications
  • Illumination: 5-channel solid-state LED light engine
  • Wide Field of View: 5-channel fluorescence imaging
  • Objective Lens: Single-position objective lens (10x, 20x)
  • Camera: 1 camera for photometric measurements
  • Brightfield: Brightfield imaging with white light
Measurement
Applications

CellInsight CX5 is a high-content analysis instrument capable of extracting quantitative data from cell populations. It is equipped with five multiplexable excitation wavelengths for fluorescence and white light illumination for colorimetric samples. The system also includes an Orbitor RS2 plate handler. At Medford Corporation, it is used for digital soft agar colony formation assays.